Chromosomes and C-banding karyotypes of Chorthippus brunneus huabeiensis Xia et Jin and Chorthippus minutus Zhang were studied for the first time, which respectively collected in Songhua Lake, Jilin Province China and Daqinggou, Inner Mongolia China. In the males, the chromosome numbers of these two species was found to be 2n (♂) =17, with three metacentric pairs and five telocentric pairs, and X chromosomes are telocentric. This genus has the basic Orthopteran sex determining mechanism XX♀/XO♂. All the chromosomes possess centromeric C-bandings. The differences of these two species are also very remarkable, such as genome formula, terminal bandings, medial bandings and content of heterochromatin.